ABSTRACT
La enfermedad neurológica bovina genera altas pérdidas económicas en las ganaderías y algunos de sus agentes etiológicos son zoonóticos, hechos que hacen prioritario su estudio. El objetivo del trabajo fue identificar antígenos del virus de la rabia y del herpes virus bovino-5 (BoHV-5) en muestras de encéfalos de bovinos archivados como casos de enfermedad neurológica con diagnóstico no conclusivo. Se seleccionaron 10 muestras del laboratorio del Instituto Colombiano Agropecuario -ICA- de Montería. Los tejidos fueron analizados mediante histopatología e inmunohistoquímica; para esta última técnica fueron utilizados tres anticuerpos: dos contra antígenos del virus de la rabia a una dilución de 1:200 y uno contra BoHV-5 a una dilución de 1:100. Histológicamente en nueve casos se observaron lesiones que variaron de tipo y severidad; la identificación de antígenos del virus de la rabia se demostró en 20% (2/10) de los casos y en ningún caso se demostró la presencia BoHV-5. Se discute sobre los resultados histopatológicos e inmunohistoquímicos y se concluye la importancia de asociar los hallazgos de las dos técnicas para establecer la etiología de la enfermedad neurológica, especialmente en casos con diagnóstico inconcluso.
Bovine neurological disease generates high economic losses in herds and some of its etiological agents are zoonotic, situations that make priority their study. The aim of this study was to identify antigens rabies virus and bovine herpesvirus-5 (BoHV-5) in cattle brain samples archived as cases of neurological disease with no conclusive diagnosis. 10 samples were selected from laboratory of the Colombian Agricultural Institute -ICA- at Monteria. Tissues were analyzed by histopathology and immunohistochemistry techniques. For immunohistochemical analysis, three antibodies were used: two against antigens of rabies virus at a dilution of 1:200, and one against antigens of BoHV-5 at a dilution of 1:100. Histologically lesions i nine cases lesions that varied in type and severity were observed; antigens detection of rabies virus could be demonstrated by immunohistochemistry in 20% (2/10) of cases and could not be demonstrated in any case the presence BoHV-5. We discuss on the histopathological and immunohistochemical finding, it is concluded on the importance of perform association of the two techniques to assist the establishing of the neurological disease etiology, especially in case without diagnostic.
ABSTRACT
El objetivo de este trabajo fue discutir sobre los hallazgos clínicos, los exámenes de patología clínica realizados y su utilidad como indicadores de pronóstico en un paciente equino hembra con hígado graso. Se describe el caso de una yegua de raza criollo colombiano que ingresó al servicio de consulta externa del Hospital Veterinario Diego Villegas Toro de la Universidad de Caldas con signos de tránsito intestinal lento y cólico. Previamente, el veterinario remitente inició un tratamiento con fluidos y aceite cristal oral sin resultados satisfactorios. Se realizó un examen físico completo y pruebas paraclínicas (creatinina, BUN, albúmina, fibrinógeno, fosfatasa alcalina [FA], gamma glutamiltransferasa [GGT], alanina aminotransferasa [ALT], aspartato aminotransferasa [AST] y análisis de orina) que sugirieron disfunción hepática y compactación del colon mayor. Se instauró un tratamiento dirigido a favorecer el peristaltismo y la eliminación fecal y se dispensó soporte para coadyuvar a la función hepática. Durante la hospitalización se observaron signos clínicos compatibles con falla hepática, encefalopatía e compactación grave; finalmente, el equino murió. Durante la necropsia se confirmó compactación del colon mayor a nivel de la flexura esternal y peritonitis. El diagnóstico histopatológico fue hígado graso y peritonitis. Se concluye que la falla hepática puede asociarse con compactación del colon.
The aim of this work was discuss on clinical findings, clinical pathology tests and their usefulness as prognostic indicators in a female equine patient with fatty liver. It is described a case in a native Colombian mare that entered the outpatient service at veterinary hospital Diego Villegas Toro of the University of Caldas with slow intestinal transit signs and mild colic. Previously the sender veterinarian began treatment with intravenous fluids and oral mineral oil oral without satisfactory results. It was performed a complete physical examination and paraclinical tests (creatinine, BUN, albumin, fibrinogen, alkaline phosphatase [AP], gamma glutamyl transferase [GGT], alanine suggestive of liver dysfunction and compaction of the large colon. It was established a treatment addressed to promote peristalsis, fecal elimination and support liver function. During the hospitalization of the patient, were observed different clinical signs consistent with liver failure, encephalopathy and severe compaction; the equine died. During the necropsy was confirmed the large colon compaction in the sternal flexure and peritonitis. The histopathological diagnosis was fatty liver and peritonitis. It is concluded that liver failure may be associated with compaction of the large colon. aminotransferase [ALT], aspartate aminotransferase [AST] and urinalysis) that were suggestive of liver dysfunction and compaction of the large colon. It was established a treatment addressed to promote eristalsis, fecal elimination and support liver function. During the hospitalization of the patient, were observed different clinical signs consistent with liver failure, encephalopathy and severe compaction; the equine died. During the necropsy was confirmed the large colon compaction in the sternal flexure and peritonitis. The histopathological diagnosis was fatty liver and peritonitis. It is concluded that liver failure may be associated with compaction of the large colon.